CloneID: 33G5
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing mice with CD117.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P10721
Specificity Statement: The antibody is specific for CD117. CD117 is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.
Application Notes (Clone): The antibody specifically detected CD117 protein in Western blot analysis. The antibody 33G5 and the commercial antibody YR145 were used to stain gastrointestinal stromal tumor tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 33G5 was higher than the commercial antibody. The antibody and the commercially available antibody YR145 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113621064A).
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