CloneID: N418
Heavy Chain modification: Fc Silent™
Antigen Long Description: Hamster spleen cells that had been primed to mouse spleen dendritic cells
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P20702
Specificity Statement: The antibody binds to mouse and human CD11c.
Application Notes (Clone): This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory responses. The binding of this antibody to its target was originally evaluated via FACS (Metlay et al., 1990; PMID: 2185332). This antibody was used for FC (Liu et al., 1998; PMID: 9723687). This antibody was used in vivo as a tool to target the delivery of a model antigen specifically to dendritic cells in mice, resulting in rapid and high antibody response (Wang et al., 2000; PMID: 10639168). Conjugating this antibody to synthetic peptides enhanced their immunogenicity (Berry et al., 2003; PMID: 10639168). This antibody was successfully used in an IF assay (AbAb internal results).
Deutsch
English
