CloneID: 5c8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with D1.1 cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P29965
Specificity Statement: This antibody is specific for human CD40 ligand.
Application Notes (Clone): The original version of this antibody was used to catalyse disfavoured endo-tet cyclization reactions of trans-epoxy alcohols (Gruber et al., 1999; PMID:10353817). The structure of the fab version of the antibody was determined using x-ray crystallography (Gruber et al., 1999; PMID:10353817, Karpusas et al., 2001; PMID:11525169). The original antibody was used for flow cytometry on D1.1 cells. It only showed binding to D1.1 cells but not to B2.7 cells. This antibody was used for immunoprecipitation on Jurkat cell lysates and precipitated a 30kDa protein. This antibody also shows the ability to inhibit D1.1 cell induced B cell activation (Lederman et al., 1992; PMID:1348081). The half life of this antibody in cynomolgus monkeys was measured. The data suggested a half life between 300-500 hours. This antibody was tested in vivo on cynomolgus monkeys. When first given this antibody and then performing a tetanus toxoid challenge it decreased antibody titres (Gobburu et al., 1998; PMID:9694951).
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