CloneID: OX-45
Antigen Long Description: OX-45 was prepared by immunizing BALB/c mice with rat T blasts prepared by stimulating purified T helper (TH) cells with allogeneic irradiated rat spleen cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P10252
Specificity Statement: OX-45 binds specifically to CD48 on rat thymocytes, T and B cells and endothelium but not LFA.3. Directly competes with CD46 for binding the target epitope. CD48 is a CD2 ligand and is expressed on the surface of all hematopoietic cells and endothelium. May be involved in the facilitation of interactions between activated lymphocytes. Likely to be involved in T cell activation.
Application Notes (Clone): Describes the use of OX-45 in blocking the primary mixed lymphocyte response (MLR)and the secondary response of T lymphocytes to the soluble antigen DNP-BGG. It also showed that OX-45 did not have any effect on the cytotoxic activity of specific (CTL) or non-specific (NK) killer cells or on proliferative responses stimulated by lectins or oxidative mitogenesis.
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