CloneID: 5D7
Heavy Chain modification: Fc Silent™
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P06127
Specificity Statement: This antibody binds human CD5, which is a surface glycoprotein that act as a receptor in regulating T-cell proliferation. CD5 is a good immunohistochemical marker for T-cells. This antibody also cross reacts with cynomolgus monkey CD5.
Application Notes (Clone): The binding of this antibody to human and cynomolgus monkey PBMCs was studied using flow cytometry. The human IgG1 version of this antibody mediates significant ADCC activity against the CLL cell line Wac3CD5, using purified NK cells as effector cells. Anti-RSV IgGI was used as an isotype control. In a caspase activity assay this antibody showed capability of inhibiting survival of both CLL and ALCL tumor cells by mediating apoptosis against these cells. Humanized version of this antibody is available on request (US20080254027).
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