CloneID: 186 2G9
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by immunizing a mouse with CD50.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P32942
Specificity Statement: This antibody is specific for human CD50.
Application Notes (Clone): The original version of this antibody was used to immunophenotype mast cells through flow cytometry, while studying the cell surface markers of human skin mast cells. (Ghannadan et al., 1998; PMID:9764855). This antibody was used to block CD50, to determine the effect of the CD50 protein on PMN attachment (Sud'ina et al., 2001; PMID:11672437). This antibody was able to stain CD50 in a immunohistochemistry experiment on psoriasis tissue. This experiment was performed to study the mechanism of action of methotrexate (Yazici et al., 2005; PMID:16215763).
Deutsch
English
