CloneID: OX-52
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q812A4
Specificity Statement: This antibody is specific for rat CD6, which is largely restricted to cells of the T-lymphocyte lineage.
Application Notes (Clone): This antibody recognizes rat CD6, which is largely restricted to cells of the T-lymphocyte lineage, and, as a result, this antibody can be used to identify such cells in tissues or cell suspensions (Robinson, 1986). In cell suspensions prepared from lymph node, TDL, thymus and spleen, exclusively T cells are labelled. Using the immunoperoxidase technique on cryostat tissue sections, staining is mainly confined to the T-cell areas of the spleen, lymph nodes and Peyer's patches, as well as all thymocytes. However, occassional positive cells have been observed in the B cell areas of lymphoid organs (Robinson, 1986), and consequently this antibody is used in conjunction with other markers, such as OX-19, to identify T cells.
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