CloneID: OX-85
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was prepared by immunizing 8 –12-week-old BALB/c mice with purified rat CD62L-CD4
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P30836
Specificity Statement: OX-12 binds to rat antibody kappa light chains - has preference for IgKa allotype.
Application Notes (Clone): This antibody binds to CD62L expressed on rat lymphocytes, and has been used to show that recombinant soluble rat CD62L-CD4, to which it also binds, is likely to be correctly folded (Nicholson, 1998). This antibody also labels, and can therefore be used to fractionate, the subpopulation of rat CD4+CD8- thymocytes that expresess L-selectin (Seddon, 1996). Cells with this phenotype are able to protect rats from diabetes upon adoptive transfer, as well as providing B cells with help for secondary antibody responses and mediating local graft-versus-host reactions.
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