Anti-Cholera enterotoxin B subunit (A9 (LCTA9))

Anti-Cholera enterotoxin B subunit [A9 (LCTA9)], Recombinant, IgG1-Fc Fusion, Human

CloneID: A9 (LCTA9)

Antigen Long Description: The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas.

Origin Pub PMID: 17165813

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: P01556

Specificity Statement: This antibody binds the B subunit of the cholera toxin.

Application Notes (Clone): The binding specificity of this antibody towards cholera toxin was checked using ELISA (PMID:17165813). SPR was used to determine the dissociation constant (Kd) of VHH A9; the Kd was determined to be 77 ± 5 nM (PMID: 23385744). It was reported that two point mutations (N27D and R71I) help stabilize A9 wild-type form which has a relatively low melting point (PMID: 31342597).

• Artikelnummer: ABAAb02809-10.159-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab02809-10.159-BT
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Recombinant
• Methode: ELISA, Super-Resolution Microscopy
• Isotyp: IgG1-Fc Fusion
• Wirt: Human