CloneID: AE3
Antigen Long Description: The original antibody was raised by immunizing a mouse with human epidermal keratins from P3 x 63 Ag8 cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for cytokeratin type 2.
Application Notes (Clone): The original version of this antibody was used for immunohistochemistry on human corneas (Haug et al., 2013; PMID:22429721), on human sentinel lymph node tissue (Atinkaya et al., 2005; PMID:16000990), and on tissue from rats bearing mammary tumours (Esendagli et al., 2009; PMID:18987061), and on lichen amyloidosis and macular amyloidosis tissue (Apaydin et al., 2004; PMID:15096140). Further this antibody was used for a western blot on DE-E, DE-F and DE-SF cell line lysates (Chang et al., 2010; PMID:19748110). This antibody was used for immunofluorescence on breast cancer cell lines MCF7, MDA-MB-231, SKBR3, SUM159, and SUM1315 and a hepatocellular carcinoma cell line HepG2 (Balic et al., 2011; PMID:20861793).
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