CloneID: LH7.2
Antigen Long Description: The original antibody was raised by immunizing a mouse with 1% Nonidet P40 extract of trypsinized cultured human keratinocytes.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q02388
Specificity Statement: This antibody is specific for amino acids 252 to 361 of Collagen alpha-1(VII) chain.
Application Notes (Clone): The original antibody was used for western blotting on overlapping recombinant type VII collagen fusion proteins and pepsinized human type VII (Lapiere et al., 1994; PMID:7530994), and on type VII collagen fusion proteins (Tanaka et al., 1994; PMID:7524608). Further this antibody was used for an ELISA on non-overlapping type VII collagen fusion proteins encompassing both non- collagenous amino- and carboxyl-terminal domains and the collagenous helical domain (Lapiere et al., 1994; PMID:7530994). This antibody was used for immunohistochemistry on normal adult human skin freshly obtained from two different sites (abdomen and arm) (Shimizu et al., 1990; PMID:2354110), on different human tumours (Wetzels et al., 1991; PMID:1867328), and on normal human tissue (Heagerty et al., 1986; PMID:3527251).
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