CloneID: 14D09
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P61073
Specificity Statement: This antibody is specific for CXCR4.
Application Notes (Clone): The binding of the original version of this antibody (VHH) to CXCR4-expressing viral lipoparticles was characterized in an ELISA. Furthermore, it was analyzed for its ability to compete with the ligand CXCL12 (or SDF-1a) for receptor binding by displacement of biotinylated SDF-1 on Caki-CXCR4 cells in flow cytometry. This antibody exhibited medium affinity to its target (EC50/kD of 1 < x ≤ 10 nM) and a ligand inhibition (IC50 1 < x < 10 nM). In its bi-specific construction with clone 57A07, it served a functional role (US20220242958).
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