CloneID: Clone 3
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was isolated from a IgM-derived scFv phage antibody library created from the peripheral blood lymphocytes of 20 non-immunized doners. The specific clone was isolated by panning the library against digoxigenin.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications.
Application Notes (Clone): The binding specificity of this antibody for digoxigenin was checked using ELISA. This antibody did not bind digoxigenin coupled by a methylcarbonyl (Dig-Met) or succinyl (Dig-Suc) type linker to BSA (PMID: 9305722).
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