CloneID: BG4
Antigen Long Description: DNA/RNA G-quadruplex.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: n/a
Specificity Statement: Binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures.
Application Notes (Clone): This antibody has been shown (by ELISA) to bind selectively to G-quadruplex forming DNA and RNA structures. Please that Fab-based versions as well as scFvs, with and without Flag-tag are available, which needs to be considered when replicating published methods. Tsai et al. (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4 was shown to have weak cross-reactivity with iMotif structures (2018, PMID: 29686376). iMotif structures can be detected selectively using our anti-iMotif antibody Ab01462.
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