CloneID: F24F2
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with B. licheniformis γDPGA in combination with CD40 agonist antibody.
Buffer Composition: PBS only.
Specificity Statement: This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d-glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Application Notes (Clone): This antibody was used for development of a quantitative capture ELISA for PDGA (PMID: 18195035). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008). The HRP or Alexa Fluor 555 labelled antibody was also used in the immunohistochemical staining of γDPGA in mouse liver and spleen tissues after they were injected with 500, 100, or 20µg of γDPGA and tissues were harvested on days 1, 2, 4, and 8 (PMID: 18195035). This antibody was also used in the cellular localization of γDPGA in mouse liver (PMID: 18195035).
Deutsch
English
