CloneID: 12G9-2
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with Dxd-KLH conjugate.
Buffer Composition: PBS only.
Specificity Statement: This antibody binds Dxd, an anti-cancer alkaloid camptothecin analog, which is a potent DNA topoisomerase I (TOP1) inhibitor. By forming a stable ternary complex with TOP1 and DNA, it arrests the cell cycle to S phase, thereby preventing DNA replication and causing cell death. DxD is used for the generation of antibody-drug conjugates for targeted delivery of toxic materials to cancerous cells. A HER2 targeting -DxD drug conjugate (DS-8201a) shows potent antitumor activity in HER2-positive models
Application Notes (Clone): The binding reactivity of this antibody towards Dxd-KLH and Dxd-BSA conjugates was determined using ELISA. This antibody is capable of binding antibody-drug conjugates ADC-8201 and ADC029 in a competitive ELISA, wherein the antibody was trastuzumab. The binding affinity of this antibody towards Dxd-BSA was determined using surface plasmon resonance (SPR) and it was reported to be Kd= 3.38nM (CN115677857).
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