CloneID: DC1.56
Antigen Long Description: The original antibody was isolated from the peripheral blood lymphocytes of a human patient with a history of symptomatic CHIKV infection using single B cell sorting with biotinylated p62-E1 hybrid protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q8JUX5
Specificity Statement: This antibody binds the E1 protein of the Chikungunya virus (CHIKV), which is the causative agent of zoonotic disease in humans called Chikungunya. Additionally, this antibody also cross reacts with multiple arthritogenic alphaviruses like Mayaro virus (MAYV), Ross River (RRV), Sindbis virus (SINV) and also some alphavirus like Venezuelan (VEEV), Eastern (EEEV), and Western (WEEV) equine encephalitis viruses (PMID: 34416146). CHIKV belongs to the alphavirus genus of the Togaviridae family and is transmitted to human by infected mosquito bites. A CHIKV infection causes fever and severe joint pain. Other symptoms include muscle pain, joint swelling, headache, nausea, fatigue and rash. The disease mostly occurs in Africa, Asia and the Indian subcontinent.
Application Notes (Clone): The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. The binding affinity of the original IgG antibody towards E1 protein was found to be Kd = 1.75 nM (PMID: 31697791).
Deutsch
English
