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Anti-EDAR (EDAR3)
Anti-EDAR [EDAR3], Recombinant, IgG2b kappa, Rat
Artikelnummer
ABAAb00784-8.4-BT
Verpackungseinheit
1 mg
Hersteller
Absolute Antibody
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CloneID: EDAR3
Heavy Chain modification: Fc Silent™
Antigen Long Description: EDAR3 was prepared by immunizing female OVE1B mice (with the Edar gene deleted) subcutaneously with human EDAR-Fc and positive hybridoma clones were screened for binding hEDAR by ELISA.
Origin Pub PMID: 21730053
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q9UNE0
Specificity Statement: EDAR3 recognises and binds to CRD1+CRD2 of the extracellular domain of human EDAR. EDAR3 cross-reacts with EDAR derived from mouse, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Application Notes (Clone): EDAR3 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR3 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 100 ng/ml; EC50 mEDAR:Fas ~ 30 ng/ml - both were less active than DEA1-Fc). The agonistic activity was shown to be cross-reactive with EDA-deficient dogs, in which dentition was corrected when the antibody was administered intravenously at 2 and 14 days old. Lacrimation was also improved in the 2 day old dogs. Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.18 mg/kg required for half-maximal tail-hair reversion. EDAR3 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR is recognized by EDAR3 in WB under non-reducing conditions.
Heavy Chain modification: Fc Silent™
Antigen Long Description: EDAR3 was prepared by immunizing female OVE1B mice (with the Edar gene deleted) subcutaneously with human EDAR-Fc and positive hybridoma clones were screened for binding hEDAR by ELISA.
Origin Pub PMID: 21730053
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q9UNE0
Specificity Statement: EDAR3 recognises and binds to CRD1+CRD2 of the extracellular domain of human EDAR. EDAR3 cross-reacts with EDAR derived from mouse, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Application Notes (Clone): EDAR3 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR3 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 100 ng/ml; EC50 mEDAR:Fas ~ 30 ng/ml - both were less active than DEA1-Fc). The agonistic activity was shown to be cross-reactive with EDA-deficient dogs, in which dentition was corrected when the antibody was administered intravenously at 2 and 14 days old. Lacrimation was also improved in the 2 day old dogs. Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.18 mg/kg required for half-maximal tail-hair reversion. EDAR3 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR is recognized by EDAR3 in WB under non-reducing conditions.
| Artikelnummer | ABAAb00784-8.4-BT |
|---|---|
| Hersteller | Absolute Antibody |
| Hersteller Artikelnummer | Ab00784-8.4-BT |
| Verpackungseinheit | 1 mg |
| Mengeneinheit | STK |
| Reaktivität | Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken |
| Klonalität | Recombinant |
| Methode | Western Blotting, ELISA, Super-Resolution Microscopy |
| Isotyp | IgG2b kappa |
| Wirt | Rat |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
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