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Application: This kit is useful as a control in studies of targeted degradation of human EGFR on the cell surface. Any human cancer cell lines expressing EGFR and RNF43 on the cell surface can be used with this product.
Background: RNF43, or ring finger protein 43, is an E3 ubiquitin ligase that acts as a tumor suppressor to negatively regulate the WNT signaling pathway through promoting the degradation of frizzled WNT receptors. AbTACs target both tumor-associated antigens (TAAs) and RNF43 on the cell surface, promoting the ubiquitination of TAAs by RNF43 and subsequent lysosome-mediated degradation. This mechanism results in the suppression of tumor cell proliferation and may enhance the efficacy of anti-cancer therapies in tumors that overexpress TAAs. This bispecific antibody facilitates EGFR degradation by recruiting RNF43 to trigger targeted degradation. Anti-EGFR-Anti-RNF43 AbTAC harnesses the synergistic effects of RNF43, an E3 ubiquitin ligase known for its role in the protein degradation, to selectively degrade EGFR in cancer cells, making it a promising therapeutic candidate for EGFR-driven cancers.
Concentration: The volume of DMSO or drug should be typically <0.1% of the total culture volume to avoid cytotoxicity.
Contraindications: The volume of DMSO or drug should be typically <0.1% of the total culture volume to avoid cytotoxicity.
Description: The Anti-EGFR-Anti-RNF43 AbTAC Kit is designed for the targeted degradation of EGFR (epidermal growth factor receptor) induced by Anti-EGFR-Anti-RNF43 AbTAC (Antibody-based Targeted Protein Degradation) (#102492) in cancer cell lines expressing both EGFR and RNF43. Anti-EGFR-Anti-RNF43 AbTAC harnesses the synergistic effects of RNF43, a transmembrane E3 ubiquitin ligase known for its role in protein degradation, to selectively degrade EGFR in cancer cells, making it a promising therapeutic candidate for EGFR-driven cancers. This kit contains enough Anti-EGFR-Anti-RNF43 AbTAC, control antibody and Bafilomycin A1 for 10 AbTAC-mediated protein degradation tests in a 6-well plate or 20 tests in a 12-well plate using the HTC 116 cell line. Further downscaling beyond a 12-well plate is not recommended, and other cell lines may require optimization.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: EGFR, RNF43
Uniprot: EGFR: P00533, RNF43: Q68DV7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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