Anti-envelope protein (ZIKVEF2L01)

Anti-envelope protein [ZIKVEF2L01], Recombinant, scFv fragment (His)

CloneID: ZIKVEF2L01

Heavy Chain modification: ScFv

Antigen Long Description: The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.

Buffer Composition: PBS only.

Specificity Statement: The antibody binds to the E protein of Zika virus. The envelope protein forms a dimer on the smooth surface of the mature virus, and participates in receptor binding and fusion to the host.

Application Notes (Clone): The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 0.55 ug/mL and Kd= 2.04 *10^-8 M). Therefore, the antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).

• Artikelnummer: ABAAb03413-30.11-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab03413-30.11-BT
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Reaktivität: Virus
• Klonalität: Recombinant
• Methode: Western Blotting, ELISA, Flow Cytometry, Inhibition
• Isotyp: scFv fragment (His)