CloneID: ZIKVEF3S06
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody binds to the E protein of Zika virus. The envelope protein forms a dimer on the smooth surface of the mature virus, and participates in receptor binding and fusion to the host.
Application Notes (Clone): The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 77.42 ug/mL and Kd= 4.55 *10^-6 M).The antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).
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