Anti-Envelope protein (ZKA78)

Anti-Envelope protein [ZKA78], Recombinant, IgG1-LALA kappa, Human
Artikelnummer
ABAAb00780-10.16-BT
Verpackungseinheit
1 mg
Hersteller
Absolute Antibody

Verfügbarkeit: wird geladen...
Preis wird geladen...
CloneID: ZKA78

Heavy Chain modification: Fc Silenced

Antigen Long Description: mAb ZKA78 was selected from EBV-immortalized memory B cells derived from ZIKV-infected, DENV-naïve human donors based on its ability to bind Zika virus (ZIKV) Envelope protein and to neutralize ZIKV infection.

Buffer Composition: PBS only.

Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: Q91KX8

Specificity Statement: ZKA78 is specific for EDI/II domain of the ZIKV envelope (E) protein, and cross-reacts with the E protein from Dengue virus (DENV), which is highly homologous (also contains high sequence similarity with other flaviviruses such as yellow fever and west nile). ZKA78 showed significant binding (determined by ELISA) to the E proteins of ZIKV, DENV1-4 and the virus-like particles (VLPs) of DENV1-4. Flavivirus E proteins mediate fusion, with EDI being involved in conformational changes required for viral entry and EDII containing a fusion loop. ZIKV is a flavivirus that can be transmitted sexually, by the Aedes mosquito vector, or vertically to a developing fetus. Most cases of ZIKV infection are asymptomatic or manifest in mild symptoms, but in some cases ZIKV infection can result in Guillain-Barré Syndrome or congenital birth defects in developing fetuses.

Application Notes (Clone): ZKA78 is able to partially neutralize ZIKV infectivity (IC50 ~2863 ng/ml), and is capable of enhancing ZIKV/ infection of K526 cells via the antibody-dependent enhancement (ADE) effect. This antibody can also neutralize (IC50~ 266 ng/ml) and enhance (by ADE) DENV infection. Neutralization of ZIKV infection can be measured using a micro-neutralization FC-based assay, and binding/specificity to E protein can be studied by ELISA. DENV2-infected AG129 mice administered with ZKA78 results in death after 5 days.
Mehr Informationen
Artikelnummer ABAAb00780-10.16-BT
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab00780-10.16-BT
Verpackungseinheit 1 mg
Mengeneinheit STK
Reaktivität Virus
Klonalität Recombinant
Methode ELISA, Flow Cytometry, Neutralization
Isotyp IgG1-LALA kappa
Wirt Human
Produktinformation (PDF) Download
MSDS (PDF) Download