CloneID: 3B1
Antigen Long Description: A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P03420
Specificity Statement: The original antibody is specific for post fusion RSV F protein (amino acids D385, F387 and N388). F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation.
Application Notes (Clone): The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 format at 3.6 ng/mL). The binding affinity of the Fab fragment to post fusion form of F was measured by surface plasmon resonance (Kd= 68 nM).
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