Anti-Fibrin beta-chain (8E5)

Anti-Fibrin beta-chain [8E5], Recombinant, IgG1 kappa, Human
Artikelnummer
ABAAb00799-10.0
Verpackungseinheit
200 μg
Hersteller
Absolute Antibody

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CloneID: 8E5

Antigen Long Description: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic β-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).

Origin Pub PMID: 9219033

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P02675

Specificity Statement: 8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.

Application Notes (Clone): The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.
Mehr Informationen
Artikelnummer ABAAb00799-10.0
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab00799-10.0
Verpackungseinheit 200 μg
Mengeneinheit STK
Reaktivität Human
Klonalität Recombinant
Methode ELISA
Isotyp IgG1 kappa
Wirt Human
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