CloneID: PIA174
Heavy Chain modification: His-Tagged
Antigen Long Description: The original antibody was raised by isolating it from a B cell culture supernatant.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: A0A059QA82
Specificity Statement: This antibody is specific for DIII domain of fusion glycoprotein F0 from human respirovirus 3.
Application Notes (Clone): The original antibody was used for an ELISA on parainfluenza virus type 3. Further the structure of this antibody was determined using cryo-EM (Stewart-Jones et al., 2018;PMID:30420505). This antibody was used for a plaque reduction assay on Vero cells infected with parental or escape variant of human respirovirus. This antibody was used for a neutralization assay. It showed here that it disrupted the formation of the HN/F complex (Marcink et al., 2023; PMID:36763666). This antibody was used for surface plasmon resonance on LuS-N71-SpyLinked-PIV3 F (Zhang et al., 2020; PMID:33097791).
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