CloneID: 1D5
Heavy Chain modification: His-Tagged
Antigen Long Description: The original antibody was raised by immunizing a mouse with Coxsackievirus A6.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: A0A0K2BNC7
Specificity Statement: This antibody is specific for the immune-dominant antigenic site within VP1 comprising the BC, DE, EF, and HI loops of Coxsackievirus A6. It does not show the same reactivity in other Coxsackievirus variants.
Application Notes (Clone): The original antibody was used for immunohistochemistry on Coxsackievirus A6 infected mice. Further this antibody was used for an in vivo neutralization assay on BALB/c mice. The BALB/c mice were passively immunized with this antibody before being infected with Coxsackievirus A6. This significantly increased survival rates. This antibody was also used for a neutralization assay where the mouse was first infected with Coxsackievirus A6 before injecting this antibody. This also increased survival rates (Yang et al., 2016; PMID:27582066). This antibody was used for an in vitro neutralization assay, the binding of Coxsackievirus A6 to RD cells was tested and showed a decrease compared to the control. This antibody was used for an ELISA on Coxsackievirus A6 particles. The structure of this antibody was determined using Cryo-EM (Xu et al., 2017; PMID:28894095).
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