CloneID: Minimizer
Antigen Long Description: The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P42212
Specificity Statement: The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP.
Application Notes (Clone): The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881).
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