CloneID: M2A1
Heavy Chain modification: His-Tagged
Antigen Long Description: M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P02724
Specificity Statement: M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ~ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Application Notes (Clone): M2A1 can bind to M antigen of glycophorin A and can be used in ELISAs, immunoblots and modified M glycoprotein can be used for inhibition tests.