CloneID: 1C11
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with SBV strain BH80/11.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: H2AM12
Specificity Statement: The antibody is specific for the envelopment polyprotein of SBV. It binds to a conformational epitope on the apical end of the head domain near the N686 glycosylation site. Its epitope has an area of 907 A2 and is centered on the third disulfide bond, with contributions from the regions around helices h8–h10 and h12–h13.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody reacted with SBV by indirect immunofluorescence (Wernike et al., 2015; PMID: 25889366). The binding affinity of the original format of the antibody to the recombinant SBV head–stalk construct was measured by surface plasmon resonances (KD = 5.4 ± 0.3 nM). The original format of the antibody neutralized SBV on Vero cells in a focus reduction neutralization (FRNT) assay with ND50 = 0.15 ± 0.01 µM. The scFv format of the antibody was co-crystallized in complex with the SBV Gc head domain. The original format of the antibody could protect IFNAR−/− mice from lethal SBV infection in vivo (Hellert et al., 2019; PMID: 30787296).
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