CloneID: CC5-17
Antigen Long Description: The original antibody was isolated from donor "CC5", a CCHFV survivor; circulating anti-GP38 antibodies were isolated from plasma using affinity chromatography. The paired heavy and light chain sequences were obtained from proteomics and genomics analyses, and the antibody was produced by transfecting Expi293 cells (expiCHO-S cells) with 1:1 ratio of heavy chain plasmid to light chain plasmid.
Origin Pub PMID: 36435827
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for site I of CCHFV GP38 with three main determinants conferring binding; the first is LCDR3 which partially interacts with the N-terminus, the second interacts with α-helix 2 of GP38, and the third interaction is the HCDR3 which interacts with a larger hydrophobic pocket on top of GP38 and the large 20 AA flexible loop. This antibody binds to GP38 from CCHFV IbAr10200, Turkey-2004, and Hoti, but it did not engage with Aigai virus GP38. Therefore, for robust binding, the GP38 protein needs to have a glycine amino acid at position 296.
Application Notes (Clone): The binding of this antibody's original format (human IgG1) to GP38 was evaluated using ELISA. It had an association constant (Ka) of 3.9 × 105 M-1s-1 as measured by surface plasmon resonance (SPR) (Durie et al., 2022; PMID: 36435827).
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