CloneID: HCMV16
Antigen Long Description: The original antibody was generated by immunization of mice with purified HCMV strain AD169.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q6SW67
Specificity Statement: This antibody binds gpUL75 (gH) of the human cytomegalovirus, which is an 86-kDa glycoprotein. The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. It is though to bind a conformational epitope on the gH, and its bind is destroyed if the protein is denatured.
Application Notes (Clone): This antibody successfully precipitates glycoprotein H from both HCMV infected cell lysates and HCMV-gH-VAC-infected cell lysates. Cellular localization of HCMV-gH studied by immunofluorescence on recombinant vaccinia virus and HCMV-infected cells using HCMV-16. HCMV-16 strongly neutralizes HCMV infectivity in vitro (PMID: 2831402). The reshaped humanized antibody recognized cells infected with a variety of virus strains and neutralized clinical isolates of CMV as efficiently as laboratory strains in a conventional plaque reduction assay (PMID: 9207350).
Deutsch
English
