CloneID: H37
Antigen Long Description: The Fab fragment was selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an HSV lysate.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody binds to herpes simplex virus (HSV) antigens. The antibody is reactive with HSV-1 and HSV-2.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The Fab was able to specifically recognize Vero cells infected with HSV in indirect immunofluorescence (IF) assays; in particular, it recognized cells infected by either HSV type 1 (HSV-1) or HSV-2. This Fab was tested in indirect IF assays against Vero cells infected with two reference HSV strains and against Vero and HEp-2 cells infected by 72 HSV-1 and 62 HSV-2 clinical isolates. The Fab gave a strong positive fluorescent signal with the F (HSV-1) and G (HSV-2) reference strains and with all the HSV-1 and HSV-2 clinical isolates tested. The detection sensitivity with the Fab in indirect IF assays was higher overall than that provided by the type-specific murine monoclonal antibodies (Cattani et al., 1997; PMID:9163470).
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