CloneID: 9Y5G6
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain amino acid sequence '229SKEGDGGIMSVRSLSED319'.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P17693
Specificity Statement: This antibody specifically binds the amino acid sequence 'SKEGDGGIMSVRSLSED' of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor.
Application Notes (Clone): This antibody can bind human HLA-G peptide 'KEGDGGIMSVRSLSED' in an ELISA with a binding affinity of Ka= 1.5 X 10^(11) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody can also be used for the immunohistochemical detection of HLA-G5 and HLA-G6 molecules in different gastric cancer tissues using immunohistochemistry (CN113045656).
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