CloneID: M75
Antigen Long Description: The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.
Origin Pub PMID: 18041760
Buffer Composition: PBS only.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q16790
Specificity Statement: This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.
Application Notes (Clone): The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 μM (Král et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).
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