CloneID: 18F4.2C8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing mice with recombinant human IL-1 alpha.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P01583
Specificity Statement: This antibody binds human Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Application Notes (Clone): The original mouse IgG antibody binds human IL-1A with a binding affinity of Kd= 0.6 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 8.90E−11M. This antibody was used in combination with 1B12.4H4 (anti-IL-1β) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).
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