CloneID: UFKA-20
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated immunizing Balb/c mice with human IL-2.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Uniprot Accession No.: P60568
Specificity Statement: This antibody binds human IL-2. In particular, the antibody binds IL-2 dorsolaterally, with an angle of 55° counterclockwise to the vertical axis, contacting the C- and B-helices of IL-2 and thereby forming a “clamp” around the C-helix. The antibody engages IL-2 residues D84, N88, V91, E61, E68, and K64.
Application Notes (Clone): This antibody binds to Interleukin-2, a protein produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/UFKA-20 complex shows an improved selectivity towards regulatory T cells compared to IL-2 alone. IL-2/UFKA-20 can recognize the receptor IL of regulatory T cells on the base of the presence of CD25, therefore called CD25 bias. IL-2/UFKA-20 complex is able to efficiently stimulate CD25+ Treg cells in mice and rhesus macaques in vivo as well as in humans ex vivo.
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