CloneID: AbAbKRAS01
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with recombinant human KRAS G12C mutant protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit IgG antibody was made using the variable domain sequences of the original mouse IgG1 format for improved compatibility with existing reagents, assays, and techniques.
Uniprot Accession No.: P01116
Specificity Statement: This antibody binds the G12C mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Application Notes (Clone): The binding reactivity of the original antibody (mouse IgG1) antibody towards mutated KRAS (G12C), VEGFA, TIGIT, HB-EGF and HSP90 was tested using ELISA. It was reported that this antibody does not cross-react with any other molecule than mutant KRAS. This antibody specifically recognized the KRAS protein in celly lysates of human lung cancer cell line A549 and human colon cancer cell line SW480 in a western blot (CN111705065).
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