Anti-LAG-3 (Ab835)

Anti-LAG-3 [Ab835], Recombinant, IgG kappa, Rabbit

CloneID: Ab835

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: P18627

Specificity Statement: The antibody is specific for human LAG-3. The antibody does not cross react with cynomolgus LAG-3.

Application Notes (Clone): The antibody is a humanized version of the mouse antibody mab7c. The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50=0.12 nM). The binding activity of the humanized antibody was tested using the hLAG-3 positive cell line, and the results showed that the antibody was positive for hLAG-3. The binding activity of the antibody and hLAG-3 positive cells was EC50 = 0.41 nM. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.52 nM). The antibody blocked the binding of MHCII and human LAG-3 with IC50 = 2.27 nM and it prevented LAG-3 and MHCII from binding to activate human T cell activity (release IL2) (EC50=1.74ug/ml) (CN110172099).

• Artikelnummer: ABAAb03509-23.0-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab03509-23.0-BT
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Recombinant
• Methode: ELISA, Super-Resolution Microscopy
• Isotyp: IgG kappa
• Wirt: Rabbit