CloneID: Ab8438
Heavy Chain modification: Fc Silent™
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P18628
Specificity Statement: The antibody is specific for human LAG-3. The antibody cross-reacts with cynomolgus LAG-3. The antibody does not cross react with murine LAG-3.
Application Notes (Clone): The antibody is a humanized version of the mouse antibody mab84c. The humanized antibody could bind to hLAG-3+ cells, with an EC50 value that does not exceed 1.5nM. The humanized antibody prevented the binding activity of human LAG-3 and Daudi cells. The binding affinity of the humanized antibody was measured by surface plasmon resonance (Kd= 0.71 nM). The antibody was employed to design bispecific antibodies against LAG-3 and PD-L1, which antibody retained the binding activity to dual-target LAG3 and PD-1. Further, both the combination of the antibody and PD-1 antibody and the bispecific LAG-3 and PD-L1 antibody showed a good tumour inhibition effect in in vivo application (CN111848794).
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