CloneID: ADI-31798
Antigen Long Description: The original antibody was isolated from a yeast display library by two rounds of screening against human LAG-3.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P18627
Specificity Statement: This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells.
Application Notes (Clone): This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125).
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