Anti-LMP1 (H3)

Anti-LMP1 [H3], Recombinant, IgG1 kappa, Human
Artikelnummer
ABAAb00761-10.0
Verpackungseinheit
200 μg
Hersteller
Absolute Antibody

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CloneID: H3

Antigen Long Description: H3 was selected by its affinity for GST-LMP1 from a human phage display library after 4 rounds of panning.

Origin Pub PMID: 17460414

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P03230

Specificity Statement: H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187-242]).  LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor-associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.

Application Notes (Clone): H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.
Mehr Informationen
Artikelnummer ABAAb00761-10.0
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab00761-10.0
Verpackungseinheit 200 μg
Mengeneinheit STK
Reaktivität Virus
Klonalität Recombinant
Methode Immunofluorescence, Immunoprecipitation, ELISA
Isotyp IgG1 kappa
Wirt Human
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