CloneID: S20
Heavy Chain modification: Fc Silent™
Antigen Long Description: Ig-inactivated transgenic mice possessing human heavy and light Igs (strain XMG2) were immunized with heat-killed 06ad P. aeruginosa. The supernatants from the derived hybridomas were screened for IgG mAb production against the 06ad high molecular weight LPS O-side-chain using ELISA.
Buffer Composition: PBS only.
Specificity Statement: S20 binds to the polysaccharide (PS) portion of the lipopolysaccharide O side chain of a common pathogenic serogroup of P. aeruginosa, 06ad. The antibody also binds Fisher Devlin immunotype 1 P. aeruginosa, (considered part of the 06 serogroup) but there is no cross-reactivity with LPSs from other P. aeruginosa serotypes (strains IATS 011, IATS 016, 170003, and PAO1 Holloway). P. aeruginosa is a human pathogen, which poses a serious risk to individuals with weak immune systems. LPS is a serotypic marker present on the surface of the bacterium.
Application Notes (Clone): S20 human MAb is highly opsonic (complement-dependent) for the uptake and killing of live and heat-killed P. aeruginosa by fresh human PMN ( polymorphonuclear leukocytes). BALB/c mice rendered neutropenic challenged with 06ad P. aeruginosa saw 100% protection from mortality. S20 can also be used to detect 06ad LPS by ELISA.