CloneID: HK1.4
Antigen Long Description: The antibody was raised by immunizing a Lewis rat by a series of four monthly i.p. injections of approximately 30 X 10" L3 cloned CTL cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2c format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P0CW02
Specificity Statement: This antibody is specific for Ly6 and shows no cross reactivity. Ly6 shows acetylcholine receptor binding activity.
Application Notes (Clone): A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild-type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). Neonatal murine calvaria-derived osteoblasts were stained for flow cytometry using this antibody (Horowitz et al, 1994; pmid:7520861).
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