CloneID: 5H4
Heavy Chain modification: Fc Silent™
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P09603
Specificity Statement: The antibody is specific for M-CSF. The antibody binds to a linear epitope represented by ITFEFVDQE, amino acids 65-73 of M-CSF.
Application Notes (Clone): The binding properties of the original format of the antibody were analyzed using SPR analyses (Kd = 3 nM). The antibody could neutralize human M-CSF activity, as measured by a proliferation assay with M-NFS-60 cell line. Further, the Fab fragment of the antibody was able to neutralize human M-CSF activity, but with less potency. The humanized variant of the antibody was constructed (EP1913028B1).
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