Anti-Major capsid protein L1 (H16.1A)

Anti-Major capsid protein L1 [H16.1A], Recombinant, IgG1 kappa, Mouse

CloneID: H16.1A

Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with HPV-16 or HPV-18 L1 VLPs.

Buffer Composition: PBS only.

Uniprot Accession No.: P03101

Specificity Statement: This antibody is specific for a conformational epitope covering amino acids 266–297 and 339–365.

Application Notes (Clone): The fab version of this antibody was used a neutralization assay, in short both a pre- and post-virus attachment assay was performed on 293TT cells with pseudo virus. The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:25996608). This antibody was used for surface plasmon resonance on virus like particles (Towne et al., 2013; PMID:23159495). This antibody was used for an ELISA on HPV-16 L1wt VLPs. This antibody was used for a neutralization assay on HPV-16 pseudo virions, in short the antibody was incubated with HPV-16 pseudo virions, then an attempt was made to infect cervical carcinoma C33A cells with the mixture. This antibody showed some neutralization (Christensen et al., 2001; PMID:11878901).

• Artikelnummer: ABAAb04465-1.1-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04465-1.1-BT
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Virus
• Klonalität: Recombinant
• Methode: Neutralization, Super-Resolution Microscopy
• Isotyp: IgG1 kappa
• Wirt: Mouse