CloneID: LAM-2
Antigen Long Description: The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q5S3G8
Specificity Statement: This antibody specifically binds mCherry fluorescent protein.
Application Notes (Clone): This antibody is specific for mCherry and does not cross react with DsRed or TdTomato. This antibody can be used to detect mCherry fluorescent protein in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.49 nM (PMID: 25362362).
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