CloneID: NL7
Antigen Long Description: The antibody was generated by immunizing BALB/c mice with Cyt b.
Origin Pub PMID: 16563510
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P04839
Specificity Statement: The antibody recognises NADPH oxidase 2 (Epitope EKDVITGLK). The antibody cross-reacts with PDIA3.
Application Notes (Clone): The antibody detected gp91phox by western blot analysis (Burritt et al., 2003; PMID: 12794137 and Bechor et al., 2015; PMID: 25699251). This antibody was used for detection of gp91phox by flow cytometry. The specificity of the antibody for gp91phox was further confirmed by phage display epitope mapping. The antibody inhibited the NADPH oxidase activity before or during the initial stages of electron transfer (Burritt et al., 2003; PMID: 12794137).
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