CloneID: SF9
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
Origin Pub PMID: 14617357
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for a very small (29 aa long; <3 kDa) epitope of non muscle Myosin IIA, starting at aa 1536 in the full human sequence.
Application Notes (Clone): The original format of this antibody (scFv) was successfully used in a Western blot and in for in vitro immunofluorescence staining of non muscle Myosin IIA (nmMyoIIA) in HeLa cells (Nizak et al., 2003; PMID: 14617357). Precise characterization of the SF9 target was achieved, defining a very small (<3 kDa) epitope within the ~200kDa nmMyoIIA protein, revealing cross-reactivity with closely related proteins of the same and across species (Vielemeyer et al., 2010; PMID: 20727208).
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