CloneID: 32
Antigen Long Description: The antibody was generated by immunizing a llama with Zika NS1 protein, followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody binds to the NS1 protein of Zika virus. The antibody did not cross-reacted with the NS1 protein of other flaviviruses, such as Yellow fever, Dengue type 1, West Nile, and Saint Louis viruses.
Application Notes (Clone): This antibody can be used to specifically detect the Zika virus as it is not cross-reactive with other Flaviviridae. The specificity of this antibody (VHH) was confirmed by ELISA analysis. Furthermore, the antibody was employed in sandwich ELISA as the detection antibody, where it was used in a matched pair in conjunction with D6. The sandwich ELISA accurately detected Zika virus NS1 protein in different serum samples in the low-ng/mL range (Delfin-Riela et al, 2020; pmid:33317184).
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