CloneID: Nb1
Antigen Long Description: The antibody was generated by immunizing a Bactrian camel with recombinant PEDV N protein followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q07499
Specificity Statement: The antibody binds the N protein of PEDV, which is associated with viral replication, transcription and assembly.
Application Notes (Clone): The specificity of this antibody (VHH) was confirmed by ELISA analysis. Langmiur model and sensorgrams data were used for kinetic analysis of PEDV N protein and Nb1. The Ka value measured was 1.97×10^5 M−1 s−1, indicating a fast association constant. The Ka/Kd value, the equilibrium dissociation constant between Nb1 and PEDV N protein, reached up to 0.96×10^8 M (Ma et al, 2019; pmid:31526383).
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